PCR

Product Name	amaR OnePCR SuperMix	amaR OnePCR HiFI	amaR OnePCR Hotstar
Item code	SM213-0250	SM215-0250	SM216-0250
5' -> 3' Exonuclease	+	-	+
3' -> 5' Exonuclease	-	+	-
Hot Star	-	-	+
Tracking Dye	+	+	+
Fluorescence Dye	+	+	+
Remarks	Red Buffer, 1 red tracking band (Amaranth), minimize side effect of blue tracking bands, formulation optimized

 

amaR OnePCR™

Item code: SM213-0250

Size: 250 Reactions (2 X 1.25 ml)

Features

• No post-staining processing of DNA required.
• No need to prepare PCR Reagents.
• Direct loading onto your agarose gel for analysis.
• Sensitivity – High degree of sensitivity as the ethidium bromide.
• Speed – No destaining requirement.
• Compatibility – Use the blue light or UV to detect the signal.

Storage

• Store at RT up to 3 months.
• Store at 4°C up to 6 months.
• Store at -20°C up to 1 year.
• Shipping Temperature: 4°C.

Note: amaR OnePCR™ is light sensitive and should be stored and protected from light.

The amaR OnePCR™ is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. The amaR OnePCR™ is a pre-mixed solution containing Taq DNA polymerase, PCR buffer, dNTPs, gel loading dyes, and fluorescence dye. The amaR OnePCR™ which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonucle ase activity. The amaR OnePCR™, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. The amaR OnePCR™ contents red tracking dyes, provide a safe, non-toxic and non-mutagenic alternative to ethidium bromide for instantaneous band visualization, that are environmentally friendly containing no hazardous chemicals. The tracking dyes that run at 10 bp on a 1% agarose gel. The amaR OnePCR™ mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for t he addition of primer and template solutions. The reagents are provided with the sufficient amplification reactions of 20 μl each.

 

Performance test data

amaR OnePCR reagent stability test after 3 months under different storage conditions. (Exposure to blue light)
– Lane M, marker ()DM010-R500)
– Lane 1(1K), RT for 3 months
– Lane 2(1K), 4°C for 3 months
– Lane 3(1K), -20°C for 3 months
– Lane 4(2K), RT for 3 months
– Lane 5(2K), 4°C for 3 months
– Lane 6(2K), -20°C for 3 months
(Program setting was followed by provided protocols)?

amaR OnePCR™ HiFi

Item code: SM215-0250

Size: 250 Reactions (2 X 1.25 ml)

Storage

• Store at RT up to 1 months.
• Store at 4°C up to 6 months.
• Store at -20°C up to 1 year.

Note: amaR OnePCR™ HiFi is light sensitive and should be stored and protected from light.

Features

• No post-staining procession.
• No need to prepare PCR reagents.
• Direct loading onto your agarose gel for analysis.
• Sensitivity- High degree of sensitivity as the ethium bromide.
• Time Efficiency- No destaining requirement.
• Compatibility – Use the Blue Light or UV to detect the signal.
• Effective for the amplification of GC-rich targets.
• Exhibits strong 3’→5′ exonuclease activityTracking dyes

The amaR OnePCR™ HiFi is a ready-to-use PCR reaction mixture. Simply adding primers and template, the reagent will execute primer extensions and other molecular biology applications. The amaR OnePCR™ HiFi is a pre-mixed solution containing GDP-HiFi DNA polymerase, PCR buffer, dNTPs, gel loading dyes, enhancer, and fluorescence dye. It contains the fluorescence dye, which is directly detected on the blue-light transilluminator or UV epi-illuminator after the DNA electrophoresis. The GDP-HiFi DNA polymerase exhibits excellent processivity, elongation capability, and strong proof-reading activity. The elongation rate of this enzyme is approximately 2 times higher than that of Taq DNA polymerase. The elongation rate is 106-138 bases/s. and produces blunt-end PCR products. The amaR OnePCR™ HiFi contents red tracking dyes, provide a safe, non-toxic and non-mutagenic alternative to ethidium bromide for instantaneous band visualization, that is environmentally friendly containing no hazardous chemicals. The tracking dyes that run at 10 bp on a 1% agarose gel. The amaR OnePCR™ HiFi mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of the primer and template solutions. The reagents are provided with sufficient amplification reactions of 20 μl each.

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  Tracking dye

• Amaranth

amaR OnePCR™ HotStar

Item code: SM216-0250

Size: 250 Reactions (2 X 1.25 ml)

Storage

• Store at room temperature up to 1 month.
• Store at 4°C up to 6 months.
• Store at -20°C up to 1 year.
• Shipping temperature: 4°C.

Note: amaR OnePCR is light sensitive and should be stored and protected from light.

Features

• No post-staining processing of DNA required.
• No need to prepare PCR Reagents.
• Direct loading onto your agarose gel.
• Sensitivity – High degree of sensitivity as the ethidium bromide.
• Time efficiency – No destaining requirement.
• Compatibility –Use the UV light or blue light to detect the signal.
• Specificity – Reduce the non-specific amplification products, thus significantly improving the specificity of PCR  reactions

AmaR OnePCR™ HotStar is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. amaR OnePCR™ HotStar is a pre-mixed solution containing hot-start Taq DNA polymerase, PCR reation buffers, dNTPs, gel loading dyes, enhancer, and fluorescence dye. amaR OnePCR™ HotStar contains hot-start Taq DNA polymerase, which exhibits non-template–dependent terminal transferase activity that adds a 3’ deoxyadenosine to product ends, and 5’→3’ exonuclease activity (but not 3’→5’ exonuclease activity). The hot-start Taq DNA polymerase is Taq DNA polymerase complexes with a proprietary antibody that blocks activity at room temperatures. When heat to 95°C, the enzyme is restored, providing an automatic “hot start” for Taq DNA polymerase in PCR, increasing the sensitivity and specificity of PCR reaction. amaR OnePCR™ HotStar contains a red tracking dye, provide a safe, non-toxic and non-mutagenic alternative to Ethidium Bromide for instantaneous band visualization, the dye is environmentally friendly containing no hazardous chemicals. The amaR OnePCR™ HotStar contains only a single fast-running tracking dye that migrates at approximately 10 base pair in a 1% agarose gel. amaR OnePCR™ HotStar also contains the fluorescence dye, which is directly detected on BLooK LED Transilluminator (BK001) or UV epi-illuminator after the DNA electrophoresis. amaR OnePCR™ HotStar mixture is supplied at the 2X concentration to allow 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with sufficient amplification reactions of 20 μl each.

Tracking Dye

• Amaranth