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Bismuth Sulfite Agar
For selective isolation of Salmonella spp, especially for Salmonella typhi.
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Item Code: HCM059
Packaging: 500g
Usages:
For selective isolation of Salmonella spp, especially for Salmonella typhi.
Principle:
Peptone, beef extract powder provides carbon, nitrogen, vitamins and minerals; glucose to provide energy; bismuth sulfite indicator suppresses gram-positive bacteria and coliform bacteria, but does not affect the growth of salmonella; agar as culture medium coagulant.
Formulation?per liter?:
Peptone 10g
Beef extract powder 5g
Dextrose 5g
Ferrous sulfate 0.3g
Disodium hydrogen phosphate 4g
Sodium sulfite 6g
Bismuth ammonium citrate 2g
Brilliant green 0.025g
Agar 15g
Final pH7.2 ± 0.2
How to use?
1. Suspend 47.3g in 1L of distilled water, stirring heated to boiling to completely dissolve, autoclave at 121? for 15 minutes.
2. Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Packaging: 500g
Usages:
For selective isolation of Salmonella spp, especially for Salmonella typhi.
Principle:
Peptone, beef extract powder provides carbon, nitrogen, vitamins and minerals; glucose to provide energy; bismuth sulfite indicator suppresses gram-positive bacteria and coliform bacteria, but does not affect the growth of salmonella; agar as culture medium coagulant.
Formulation?per liter?:
Peptone 10g
Beef extract powder 5g
Dextrose 5g
Ferrous sulfate 0.3g
Disodium hydrogen phosphate 4g
Sodium sulfite 6g
Bismuth ammonium citrate 2g
Brilliant green 0.025g
Agar 15g
Final pH7.2 ± 0.2
How to use?
1. Suspend 47.3g in 1L of distilled water, stirring heated to boiling to completely dissolve, autoclave at 121? for 15 minutes.
2. Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
