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Brain Heart Infusion Agar
For the cultivation of fastidious and non-fastidious microorganisms.
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Item Code: HCM019
Packaging: 500g
Usages:
For the cultivation of fastidious and non-fastidious microorganisms.
Principle:
Pancreatic protein peptone and bovine heart infusion provide nitrogen, vitamins and growth factors; glucose can provide energy for a variety of bacteria; sodium chloride to maintain osmotic equilibrium; disodium hydrogen phosphate buffer.
Formulation?per litre ?:
Pancreatic peptone 10g
Sodium chloride 5g
Disodium hydrogen phosphate 2.5g
Glucose 2g
Bovine heart infusion (brain heart infusion powder) 500mL (17.5g)
Final pH 7.4 ± 0.2
How to use?
1. Suspend 36g in 1L of distilled water, heated to boiling stirring until completely dissolved, dispensing tube or flask, autoclave at 121 ? for 15 minutes.
2. Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Packaging: 500g
Usages:
For the cultivation of fastidious and non-fastidious microorganisms.
Principle:
Pancreatic protein peptone and bovine heart infusion provide nitrogen, vitamins and growth factors; glucose can provide energy for a variety of bacteria; sodium chloride to maintain osmotic equilibrium; disodium hydrogen phosphate buffer.
Formulation?per litre ?:
Pancreatic peptone 10g
Sodium chloride 5g
Disodium hydrogen phosphate 2.5g
Glucose 2g
Bovine heart infusion (brain heart infusion powder) 500mL (17.5g)
Final pH 7.4 ± 0.2
How to use?
1. Suspend 36g in 1L of distilled water, heated to boiling stirring until completely dissolved, dispensing tube or flask, autoclave at 121 ? for 15 minutes.
2. Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
