Dehydrated Culture Media

Usages?
For determination of coliform confirmatory test for multi-tube fermentation.

Principle:
Peptone provides carbon and nitrogen sources; lactose as fermentable sugars; ox bile powder and brilliant green inhibit the non-Enterobacteriaceae.

Item Code: HCM004 
Packaging Size: 500g
 
Formulation (per litre):
Peptone: 10g
Lactose: 10g
Ox bile powder: 20g
Brilliant green: 0.0133g
Final pH7.2 ± 0.2
 
How to use:

1. Suspend 40g of the product, add 1 L of distilled water, stirring heated to boiling until completely dissolved, packed in a test tube with a small inverted tube, autoclave at 121^oC for 15min,  cool to room temperature and set aside.
2. Sample handling and dilution. Slightly.
3. Transplanted LST broth tube to brilliant green lactose bile broth tube by using an inoculating loop.
4. The tube was placed in an incubator culture at 36 ± 1^oC for 24h.
5. Observe the results. Press production trachea number, check table report MPN MPN value per gram (mL) sample of coliform bacteria.

 
Quality control:
Quality control strains were inoculated and cultured at 36 ± 1^oC for 24h, results are as follows:

Bacteria name	Growth situation	Gassing
Escherichia coli ATCC25922	Good	+
Salmonella typhimurium CMCC (B) 50115	Good	-
Staphylococcus aureus ATCC6538	Suppressed	-

 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.