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Overview
Usages:
For the rapid detection and enumeration of coliform bacteria.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and traces elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibits Gram-positive bacteria; chromogenic substrate and large intestine flora β- galactosidase-glucosidase specific reaction, hydrolysis of the substrate, the release of the colour groups produce green colonies on the light yellow plate.
Formulation (per litre):
Peptone:10g
Yeast extract powder: 3g
sodium chloride:5g
sodium lauryl sulfate:0.1g
Agar:12g
Chromogenic substrate 2.7g
Final pH 7.0 ± 0.2
How to use:
For the rapid detection and enumeration of coliform bacteria.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and traces elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibits Gram-positive bacteria; chromogenic substrate and large intestine flora β- galactosidase-glucosidase specific reaction, hydrolysis of the substrate, the release of the colour groups produce green colonies on the light yellow plate.
Formulation (per litre):
Peptone:10g
Yeast extract powder: 3g
sodium chloride:5g
sodium lauryl sulfate:0.1g
Agar:12g
Chromogenic substrate 2.7g
Final pH 7.0 ± 0.2
How to use:
- Suspend 32.8g of this product, adding 1L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask without autoclaving.
- Take 25g or 25mL of the sample with aseptic procedures, added to the flask containing 225mL of sterile phosphate-buffered saline (or saline) which is shaken thoroughly homogenized with a homogenizer for 1min into a 1:10 dilution, then continue to make for 1:10 dilution. select 3 of appropriate serial dilutions, each dilution was inoculated for 2 plates.
- Pouring method: Cooled in a water bath to about 50oC, sterilized petri dish having a diameter of 90mm, 1ml samples were inoculated per dish, then poured into about 15ml of the above dissolution medium, and mix to solidify, cultivating at 37oC for 24 hours.
- Using surface inoculation: cooled to about 50oC, shake devoted to the medium in a sterile petri dish. stored in the refrigerator for a day or stored at room temperature for several days (dark, 4oC). The sample was collected via streaked method or filter method, cultivating 37oC for 24 hours.
- Observe the results.
Quality control:
This product appears light yellow after pouring into the plate, these strains were inoculated after 36 ± 1 ? 18 ~ 24h culture growth in the following table.
| Strains codes | Growth of bacteria | Features |
|---|---|---|
| Escherichia coli (ATCC25922) | Good | Green colonies |
| Citrobacter (ATCC8090) | Good | Green colonies |
| Salmonella Typhimurium (CMCC50115) | Good | Colourless colonies |
| Enterococcus faecalis (ATCC29212) | Suppressed | - |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
