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Overview
Usages:
For the rapid detection and enumeration of Escherichia coli.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and traces elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibits Gram-positive bacteria; chromogenic substrate and large intestine coli β- glucuronidase enzyme-specific reaction, hydrolysis of the substrate, the release of the colour groups produce green colonies on the light yellow plate.
Packaging size:
1000ml
Formulation (per litre):
Peptone:15.0g
Yeast extract powder: 3.0g
Sodium chloride: 5.0g
Sodium lauryl sulfate:0.1g
Agar: 12.0g
Chromogenic substrate 6.5g
Final pH 7.0 ± 0.2
How to use:
- Suspend 41.6g of the product, adding 1.0L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into the flask, autoclave at 115 oC for 10minutes.
- Take 25.0g or 25.0ml of the sample with sterile procedures, added to the flask containing 225.0mL of sterile phosphate-buffered saline (or saline), shaken thoroughly homogenized with a homogenizer or a 1:10 dilution of 1min solution, diluted 1:10 and then continue to select the appropriate serial dilutions of three, the two plates inoculated with each dilution, poured dissolved by heating and cooled to about 45 oC medium.
- Observe the results.
Quality Control
This product appears light yellow after the pouring on the plate, these strains were inoculated after 36 ± 1 oC 18 ~ 24h culture growth in the following table.
| Bacteria name | Growth situation | Features |
|---|---|---|
| Escherichia coli (ATCC25922) | Good | Green colonies |
| Citrobacter (ATCC8090) | Good | Colourless colonies |
| Salmonella Typhimurium (CMCC50115) | Good | Colourless colonies |
| Enterococcus faecalis (ATCC29212) | Suppressed | - |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
