Column

Item code: SN023-0100
Quantity: 100 reactions

Sample: 300 μl of the whole blood
200 μl of the buffy coat
Mammalian cells (up to 1×10?)
Fungus cells (up to 1×10?)
Bacterial cells (up to 1×10?)
Format: spin column
Column capacity: up to 50 μg
Operation time: within 60 minutes

The Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) is designed specifically for genomic DNA isolation from the whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungus. This unique buffer system ensures genomic DNA with high yield and good quality from samples. The spin column is designed to purify or concentrate genomic DNA products which have been previously isolated using buffers. The entire procedure can be completed in 1 hour without phenol/ chloroform extraction. Purified genomic DNA is suitable for use in PCR or other enzymatic reactions.

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Applications

• Gene cloning.
• PCR.
• Southern blotting.
• SNP genotyping.

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Quality Control
The quality of the Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) is tested on a lot-to-lot basis to ensure consistent product quality.

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Features

• Fast procedure and delivering high-quality genomic DNA.
• Ready-to-use genomic DNA for high performance in any downstream application.
• Highly purified and high yield genomic DNA can be extracted from various samples.
• Optimized lysis buffer for the efficient lysis.
• Designed to rapidly purify high-quality DNA using spin column format.

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Kit Contents

Contents	Size
Buffer CR	100 ml
Buffer CC	35 ml
Buffer CB	45 ml
Buffer W1	45 ml
Buffer W2 (Add ethanol)	15 ml (60ml)
Buffer BE	10 ml
CC Column	50 pieces x 2
Collection Tube	50 pieces x 2