Chromogenic Media

Item code: CRM009

Usage:
For separation and identification of Vibrio cholera in aquatic products and food poisoning sample.
 
Principle:
Peptone and yeast extract powder to provide a nitrogen source, vitamins, amino acid and a carbon source; sucrose as fermentable sugars; most non-bacteriostatic agents inhibit Vibrio cholerae bacteria, sodium chloride osmotic pressure balance can be maintained; agar as culture medium coagulant; pigment and Vibrio cholerae enzyme has specific reaction, hydrolysis of the substrate, the release of the color groups to produce yellowish green on the plate - blue-green colonies.
 
Formulation (per liter):
Peptone: 15g
Yeast extract powder:5g
Sucrose: 10g
Sodium chloride:5g
Bacteriostatic agent :5g
Agar :13g
Pigment:3.1g
Final pH 9.2 ± 0.1
 
How to use:
1.Suspend 56.1g dissolved in 1000ml of distilled water, according to the proportion of the amplified or reduced. Heated to boiling stirring until completely dissolved, without autoclaving, cooled to about 50 ?, pour into sterile petri dish.
2.Take 25 g (mL) of test sample with aseptical procedure, adding 225ml of alkaline peptone water , with a rotating blade homogenizer at 8 000 r / min homogenized 1 min, or slap slap type homogenizer 2 min, to prepare a uniform dilution 1:10. If no homogenizer, then samples were placed in a sterile mortar and ground, and then placed in 500 mL of sterile container, add 225 mL of alkaline peptone water and thoroughly shaken.
3.Enrichment: 1:10 dilution above were cultured at 36 ? ± 1 ? for 6h ~ 8h, if necessary do secondary enrichment.
4.Cross and separation on Vibrio cholerae plate and incubated at 36 ? ± 1 ? for 18 h ~ 24 h.
5.Further confirmation of Vibrio cholerae hypothetical validation test, such as oxidase, Gram staining.
 
Quality control:
 This product appears light yellow after  pouring into plate, these strains were inoculated after 36 ± 1 ? cultured for 18-24 hours growth in the following table:
Bacteria name                 inoculum (cfu / plate)  growth situation   feature
Vibrio cholerae non-VBO 01      30--300              good      green - blue and green
Escherichia coli ATCC 25922      5000              not growth        --
Vibrio vulnificus ATCC27562      5000              not growth        --
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
 
Specifications: Configurable 1L of Medium .