Usages:
For isolation and enumeration of coagulase-positive staphylococci in foodstuffs.
Principle:
Tryptone, beef extract powder and yeast extract powder provides carbon and nitrogen sources, vitamins and growth factors; sodium pyruvate and glycine to stimulate the growth of Staphylococcus aureus; lithium chloride and potassium tellurite inhibit the non-staphylococcal microorganisms; containing lecithinase staphylococcal colonies produce degradation yolk make a transparent circle, while the role of the lipase produced an opaque precipitate ring; coagulase-positive staphylococci can restore potassium tellurite and produce black colonies; agar is a medium coagulant.
Formulation(per liter):
Pancreatic Digest of Casein 10g
Beef Extract 5g
Yeast Extract 1g
Sodium Pyruvate 10g
Glycine 12g
Lithium Chloride 5g
Agar 20g
Final pH7.0 ± 0.2
How to use it
1. Suspend 63g in 950ML of distilled water, stirring heated to boiling, autoclave at 121
oC for 15 minutes.
2. Diluted and treated samples.
Quality control?
| Item |
The name and number of strain |
Growth |
Colony Color |
| 1 |
Staphylococcus aureus ATCC6538 |
Good |
black |
| 2 |
Staphylococcus epidermidis CMCC (B) 26069 |
Good |
Green-blue |
| 3 |
Escherichia coli ATCC25922 |
Not growth |
-- |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
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