For isolation for Salmonella (except S. typhi).
Peptone provides the carbon and nitrogen; sodium chloride maintains osmotic equilibrium; lactose, sucrose, sugars that can be fermented; phenol red indicator in yellow when pH6.8, pH8.1 pink, acid-producing fermentable sugars colonies yellow, not red colonies fermentable sugar; brilliant green against Gram-positive bacteria and most non-Salmonella Gram-negative bacilli.
Beef extract powder 5g
Yeast extract powder 3g
Disodium hydrogen phosphate 1g
Sodium dihydrogen phosphate 0.6g
Phenol red 0.09g
Brilliant green 0.005g
Final pH7.0 ± 0.2
How to use?
1. Suspend 54.7g in 1 L of distilled water, stirring heated to boiling until completely dissolved, dispensing flask, 121 ? autoclaves for 15min.
2. Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.