Selenite Cystine Broth

Usages:
For selective enrichment of Salmonella

Principle:
Peptone provides carbon and nitrogen sources to meet the needs of bacterial growth; lactose is fermentable sugars; selenite, sodium hydrogen inhibit Gram-positive bacteria and gram-negative enterobacteria most non-Salmonella; phosphate-buffered agent; L- cystine as a reducing agent.

Item Code: HCM011
Packaging size: 500g
 
Formulation?per litre):
Peptone: 5g
Lactose:4g
Sodium selenite: 4g
Disodium hydrogen phosphate: 10g
L- cystine: 0.01g
Final pH 7.0 ± 0.2          
 
How to use:

1. Weigh 23g of the product, adding 1 L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing flask, cooled to room temperature.
2. Pipette 10mL of pre-enrichment sample broth or 25mL and transferred species in the liquid sample flask in a sterile environment.
3. Place into the incubator, cultured at 36 ± 1^oC for 18-24h.
4. Observe the results.

Quality control:
Quality control strains were inoculated,and cultured at 36 ± 1 ? for 18-24h, results show as follows: