The ELISA Principles & Troubleshooting


The ELISA Principles & Troubleshooting

The enzyme-linked immunosorbent assay (ELISA) is a staple in many laboratories. ELISA is an antibody-based reaction that allows the detection and quantitation of numerous analytes with the same basic assay process. The reaction uses a solid surface, typically a microplate, to immobilize the analyte. Once immobilized, unwanted materials can be removed by washing and the remaining analyte quantitated. The assay technology uses antibodies in conjunction with enzyme-catalyzed amplification to provide high specificity and high sensitivity. While relatively simple in theory, there is significant room for errors that can cause ambiguous and inconsistent results.

Here we describe the basic process steps of ELISA, best laboratory practices along with troubleshooting tips for common problems. This information will be of interest to individuals involved in developing, manufacturing, and using ELISA based immunoassays.

In this webinar, we will cover: 

  • Introduction to the principles of ELISA
  • Comparison of the most common types of ELISA & when to choose them
  • What happens in an ELISA? - General ELISA workflow
  • ELISA Troubleshooting - Common pitfalls (and how to avoid them)


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