DNA-free gene editing can be achieved by using purified Cas9 enzymes with gRNA and transfecting them directly into your cells or protoplasts of interest. GenCRISPR™ Cas9 nuclease is the recombinant Streptococcus pyogenes Cas9 protein purified from E. coli and can be used for genome editing by inducing site-specific double stranded breaks in double stranded DNA.
The GenCRISPR™ Gene editing technology has been licensed-in from the Broad Institute. The activity of all GenCRISPR™ Cas9 nucleases has been validated by in-vitro or in-vivo assays. The bioactivity data for all enzymes is included in the protocol document.
The many unknown aspects surrounding a genome's functions and regulatory framework make it difficult to estimate the impact of a gene-editing strategy. GenCRISPR™ ready-to-use kits simplify Cas9-mediated gene-editing, and help you estimate and measure the effectiveness of your gene-editing experiments.
CRISPR/Cas9 system is a powerful gene editing tool to modify target genes. Successful applications of the CRISPR Cas9 technology have been reported across single cell and multi-cell organisms, from human cells to plants such as maize and cotton. The approach is simple to implement using the Cas9 enzyme and a gRNA molecule, and can be applied in a DNA-free system. The Cas9 enzyme originating from Streptococcus pyogenes (SpCas9) is the most widely studied, and Cas9-specific antibodies are used to ascertain enzyme expression.
Key applications: Western Blotting, FACS, ELISA, IHC
GenCRISPR™ gRNA/Cas9 Plasmids| All-In-One or Dual SpCas9, Nickase & SaCas9, Constructs for gRNA Expression, SAM Constructs for Transcription Activation
GenScript licenses CRISPR technology from the Broad Institute of MIT and Harvard. GenScript offerings include the latest CRISPR plasmids and databases developed by the CRISPR pioneering Feng Zhang laboratory. Broad Institute-validated plasmids are a well-tested platform for expressing CRISPR/Cas9, and avoid instability issues in RNA-based platforms.
CRISPR/Cas9 technology is a powerful tool for creating gene knock-ins and knock-outs. Using the ribonucleoprotein (RNP) system for delivering CRISPR/Cas9 has many advantages over the traditional plasmids or virus based delivery methods.
Detectable at high levels shortly after transfection
Quickly cleared from the cell for less off-target effects
Highly efficient even in hard-to-transfect cells
Best for in vivo studies
CRISPR gRNA Library Services| GeCKO Libraries for Genome-Wide Knock-Out SAM Libraries for Genome-Scale Transcription Activation
Pooled CRISPR guide RNA libraries, or gRNA libraries, are ideal for high-throughput screening of important molecular targets. These libraries leverage the efficiency and specificity of the CRISPR gene editing technology to either knock-out gene expression or transcriptionally activate genes in the genome.
GenScript offers a variety of Broad Institute pre-validated gRNA library options, including GeCKO libraries for genome-wide knockout of human and mouse genes, and CRISPR Synergistic Activating Mediator (SAM) libraries for transcriptional activation of every gene in the human and mouse genome.
GenScript also offers fully customized gRNA libraries, with complete coverage and uniform distribution, for CRISPR knockout, CRISPRa, and CRISPRi.