For enumeration of heterotrophic bacteria in water.
Yeast extract, peptone, provide nitrogen acid protein hydrolysates, vitamins, growth factors; glucose, to fermentable sugars; soluble starch can absorb the harmful substances produced by bacteria in the recovery process; sodium pyruvate may enhance recovery of bacteria. PH buffer is potassium dihydrogen phosphate; provided over anhydrous magnesium divalent cations; agar as a coagulant.
Yeast extract powder 0.5g
Casein hydrolyzate 0.5g
Soluble starch 0.5g
Potassium dihydrogen phosphate 0.3g
Anhydrous magnesium sulfate 0.024g
Sodium pyruvate 0.3g
Final pH 7.2 ± 0.2
How to use?
1. Suspend 18.1g in 1 L of distilled water, stirring heated to boiling until completely dissolved, dispensing flask, 121 ? autoclaves for 15min.
2. Diluted and treated samples.
|The name and number of strain
||Culture Time & Temperature
|Bacillus subtilis CMCC(B)63501
Promote growth ability
|Pseudomonas aeruginosa CMCC(B)10104
: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.